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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
11/01/2018 |
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Data da última atualização: |
27/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ASCARI, I. J.; ALVES, N. G.; JASMIN, J.; LIMA, R. R.; QUINTAO, C. C. R.; OBERLENDER, G.; MORAES, E. A.; CAMARGO, L. S. de A. |
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Afiliação: |
I. J. ASCARI, UFLA; N. G. ALVES, UFLA; J. JASMIN, UFRJ; Renato Ribeiro Lima, UFLA; CAROLINA CAPOBIANGO ROMANO QUINTAO, CNPGL; G. OBERLENDER, IEF Muzambinho, MG; E. A. MORAES, Universidade Federal do Vale do São Francisco; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL. |
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Título: |
Addition of insulin-like growth factor I to the maturation medium of bovine oocytes subjected to heat shock: effects on the production of reactive oxygen species, mitochondrial activity and oocyte competence. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Domestic Animal Endocrinology, v. 60, p. 50-60, 2017. |
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DOI: |
10.1016/j.domaniend.2017.03.003 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract This study was performed to investigate the effects of insulin-like growth factor-I (IGF-I) addition to in vitro maturation (IVM) medium on apoptosis, mitochondrial membrane potential, ROS production, and developmental competence of bovine oocytes subjected to heat shock. Two temperatures (conventional: 24 h at 38.5C, or heat shock: 12 h at 41C followed by 12 h at 38.5C) and 3 IGF-I concentrations (0, 25, and 100 ng/mL) were tested during IVM. The oocytes were then fertilized in vitro, and the presumptive zygotes were cultured until reaching the blastocyst stage. There was no interaction between temperature and IGF-I concentration for any variable evaluated (P > 0.05). The addition of IGF-I did not alter the proportion of nuclear maturation, TUNEL-positive oocytes and caspase-3 activity, or blastocyst proportion on Days 7 and 8 post-fertilization. Furthermore, the total number of cells and the number of cells in the inner cell mass (ICM) in the blastocyst were not altered (P > 0.05). However, IGF-I increased (P < 0.05) the mitochondrial membrane potential and the production of ROS in oocytes and decreased (P < 0.05) the proportion of apoptotic cells in the ICM in blastocysts. Heat shock increased (P < 0.05) the proportion of TUNEL-positive oocytes and ROS production and reduced (P < 0.05) the mitochondrial membrane potential. Moreover, heat shock increased (P < 0.05) the apoptosis proportion in the ICM cells. In conclusion, supplementing IVM mediumwith IGF-I may increase the mitochondrial membrane potential and ROS production in oocytes and decrease apoptosis in the ICM in blastocysts. Heat shock for 12 h compromised oocyte developmental competence and increased apoptosis within the ICM cells of the blastocysts. MenosAbstract This study was performed to investigate the effects of insulin-like growth factor-I (IGF-I) addition to in vitro maturation (IVM) medium on apoptosis, mitochondrial membrane potential, ROS production, and developmental competence of bovine oocytes subjected to heat shock. Two temperatures (conventional: 24 h at 38.5C, or heat shock: 12 h at 41C followed by 12 h at 38.5C) and 3 IGF-I concentrations (0, 25, and 100 ng/mL) were tested during IVM. The oocytes were then fertilized in vitro, and the presumptive zygotes were cultured until reaching the blastocyst stage. There was no interaction between temperature and IGF-I concentration for any variable evaluated (P > 0.05). The addition of IGF-I did not alter the proportion of nuclear maturation, TUNEL-positive oocytes and caspase-3 activity, or blastocyst proportion on Days 7 and 8 post-fertilization. Furthermore, the total number of cells and the number of cells in the inner cell mass (ICM) in the blastocyst were not altered (P > 0.05). However, IGF-I increased (P < 0.05) the mitochondrial membrane potential and the production of ROS in oocytes and decreased (P < 0.05) the proportion of apoptotic cells in the ICM in blastocysts. Heat shock increased (P < 0.05) the proportion of TUNEL-positive oocytes and ROS production and reduced (P < 0.05) the mitochondrial membrane potential. Moreover, heat shock increased (P < 0.05) the apoptosis proportion in the ICM cells. In conclusion, supplementing IVM mediumwith IGF-I may inc... Mostrar Tudo |
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Palavras-Chave: |
Mitochondrial membrane potential; ROS production; Thermoprotection. |
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Thesaurus Nal: |
DNA fragmentation; heat stress. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02716naa a2200277 a 4500
001 2085044
005 2023-01-27
008 2017 bl uuuu u00u1 u #d
024 7 $a10.1016/j.domaniend.2017.03.003$2DOI
100 1 $aASCARI, I. J.
245 $aAddition of insulin-like growth factor I to the maturation medium of bovine oocytes subjected to heat shock$beffects on the production of reactive oxygen species, mitochondrial activity and oocyte competence.$h[electronic resource]
260 $c2017
520 $aAbstract This study was performed to investigate the effects of insulin-like growth factor-I (IGF-I) addition to in vitro maturation (IVM) medium on apoptosis, mitochondrial membrane potential, ROS production, and developmental competence of bovine oocytes subjected to heat shock. Two temperatures (conventional: 24 h at 38.5C, or heat shock: 12 h at 41C followed by 12 h at 38.5C) and 3 IGF-I concentrations (0, 25, and 100 ng/mL) were tested during IVM. The oocytes were then fertilized in vitro, and the presumptive zygotes were cultured until reaching the blastocyst stage. There was no interaction between temperature and IGF-I concentration for any variable evaluated (P > 0.05). The addition of IGF-I did not alter the proportion of nuclear maturation, TUNEL-positive oocytes and caspase-3 activity, or blastocyst proportion on Days 7 and 8 post-fertilization. Furthermore, the total number of cells and the number of cells in the inner cell mass (ICM) in the blastocyst were not altered (P > 0.05). However, IGF-I increased (P < 0.05) the mitochondrial membrane potential and the production of ROS in oocytes and decreased (P < 0.05) the proportion of apoptotic cells in the ICM in blastocysts. Heat shock increased (P < 0.05) the proportion of TUNEL-positive oocytes and ROS production and reduced (P < 0.05) the mitochondrial membrane potential. Moreover, heat shock increased (P < 0.05) the apoptosis proportion in the ICM cells. In conclusion, supplementing IVM mediumwith IGF-I may increase the mitochondrial membrane potential and ROS production in oocytes and decrease apoptosis in the ICM in blastocysts. Heat shock for 12 h compromised oocyte developmental competence and increased apoptosis within the ICM cells of the blastocysts.
650 $aDNA fragmentation
650 $aheat stress
653 $aMitochondrial membrane potential
653 $aROS production
653 $aThermoprotection
700 1 $aALVES, N. G.
700 1 $aJASMIN, J.
700 1 $aLIMA, R. R.
700 1 $aQUINTAO, C. C. R.
700 1 $aOBERLENDER, G.
700 1 $aMORAES, E. A.
700 1 $aCAMARGO, L. S. de A.
773 $tDomestic Animal Endocrinology$gv. 60, p. 50-60, 2017.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Agroindústria Tropical. Para informações adicionais entre em contato com cnpat.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agroindústria Tropical. |
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Data corrente: |
23/10/2007 |
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Data da última atualização: |
05/02/2009 |
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Tipo da produção científica: |
Artigo em Anais de Congresso / Nota Técnica |
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Circulação/Nível: |
-- - -- |
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Autoria: |
ARAÚJO, J. B. C.; VASCONCELOS, H. E. M.; PIMENTEL, J. C. M.; PAIVA, F. F. A.; MARINHO, F. de A.; MATTOS, A. L. A. |
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Afiliação: |
João Bosco C. Araújo, CNPAT; Helenira Ellery M. Vasconcelos, CNPAT; José Carlos M. Pimentel, CNPAT; Francisco Fábio de A. Paiva, CNPAT; Francisco de A. Marinho, CNPAT; Adriano Lincoln A. Mattos, CNPAT. |
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Título: |
Elementos constitutivos de capital social nos projetos de assentamento Che Guevara e Santa Bárbara, localizados no Estado do Ceará. |
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Ano de publicação: |
2007 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE SISTEMAS DE PRODUÇÃO, 7., 2007, Fortaleza-CE, Agricultura familiar, políticas públicas e inclusão social: anais. Fortaleza: Embrapa Agroindústria Tropical: BNB, 2007. |
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Idioma: |
Português |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00716naa a2200169 a 4500
001 1426635
005 2009-02-05
008 2007 bl uuuu u00u1 u #d
100 1 $aARAÚJO, J. B. C.
245 $aElementos constitutivos de capital social nos projetos de assentamento Che Guevara e Santa Bárbara, localizados no Estado do Ceará.
260 $c2007
700 1 $aVASCONCELOS, H. E. M.
700 1 $aPIMENTEL, J. C. M.
700 1 $aPAIVA, F. F. A.
700 1 $aMARINHO, F. de A.
700 1 $aMATTOS, A. L. A.
773 $tIn: CONGRESSO BRASILEIRO DE SISTEMAS DE PRODUÇÃO, 7., 2007, Fortaleza-CE, Agricultura familiar, políticas públicas e inclusão social: anais. Fortaleza: Embrapa Agroindústria Tropical: BNB, 2007.
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